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KMID : 1007520230320040517
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Food Science and Biotechnology
2023 Volume.32 No. 4 p.517 ~ p.529
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Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strain Bifidobacterium bifidum EPS DA-LAIM
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Kang Min-Joo
Jeong Hui-Jin
Kim Su-In
Shin Jae-In
Song Young-Bo
PROF WAJEEHA BEGUM
Park Hyoung-Geun
Lee Tae-Ho
Hong Su-Jin
Han Young-Sun
Lee Bong-Gyeong
Lee Ho-Jin
Park Min-Ju
Park Young-Seo
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Abstract
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Exopolysaccharide (EPS)-producing Bifidobacterium bifidum EPS DA-LAIM was isolated from healthy human feces, the structure of purified EPS from the strain was analyzed, and its prebiotic activity was evaluated. The EPS from B. bifidum EPS DA-LAIM is a glucomannan-type heteropolysaccharide with a molecular weight of 407?1007 kDa, and its structure comprises 2-mannosyl, 6-mannosyl, and 2,6-mannosyl residues. The purified EPS promoted the growth of representative lactic acid bacteria and bifidobacterial strains. Bifidobacterium bifidum EPS DA-LAIM increased nitric oxide production in RAW 264.7 macrophage cells, indicating its immunostimulatory activity. Bifidobacterium bifidum EPS DA-LAIM also exhibited high gastrointestinal tract tolerance, gut adhesion ability, and antioxidant activity. These results suggest that EPS from B. bifidum EPS DA-LAIM is a potentially useful prebiotic material, and B. bifidum EPS DA-LAIM could be applied as a probiotic candidate.
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KEYWORD
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Exopolysaccharide, Bifidobacterium bifidum, Prebiotics, Probiotics, Immunostimulatory activity
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